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OncoMatch/Clinical Trials/NCT07741435

Methylation Profile Test (Methylscape) in Body Fluids for Multi-Cancer Detection and Monitoring in Colombia

Is NCT07741435 recruiting? Yes, currently enrolling (Sep 2026).

RecruitingCentro de Tratamiento e Investigación sobre Cáncer, Luis Carlos Sarmiento AnguloNCT07741435Data as of Sep 2026Location: Colombia

DNA methylation changes occur early and broadly during carcinogenesis. Methylscape is a rapid assay that detects global DNA methylation patterns in body fluids (blood, urine, and saliva) and may detect a cancer signal and predict the cancer signal origin (CSO) from a single fluid sample, using the differential interaction between methylated and unmethylated DNA and gold nanoparticles. This prospective observational study evaluates the diagnostic performance (sensitivity and specificity) of the Methylscape test in Colombian patients with various biopsy-confirmed solid tumors compared with age- and sex-matched cancer-free (healthy) volunteers. The study is conducted in three parts: Phase 1 validates the assay in 250 patients with cancer; Sub-study 2a compares 1,500 patients with cancer against 1,500 matched cancer-free volunteers; and Sub-study 2b uses serial blood and urine sampling in 300 patients with early-stage disease to assess detection of disease relapse during follow-up, in parallel with standard imaging. The study also estimates positive and negative predictive values (PPV/NPV) and projects the budget impact and cost-effectiveness of Methylscape as a multi-cancer early detection (MCED) tool in an upper-middle-income Latin American setting.

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Extracted eligibility criteria

Cancer type

Tumor Agnostic

Breast Carcinoma

Gastric Cancer

Cervical Cancer

Colorectal Cancer

Head and Neck Squamous Cell Carcinoma

Performance status

ECOG 0–2(Ambulatory, capable of self-care)

Prior therapy

Cannot have received: anticancer therapy

Exception: surgery limited to diagnostic procedures

Prior or ongoing anticancer therapy: cancer surgery beyond that needed for diagnosis; local, regional, or systemic chemotherapy (including chemoembolization); targeted therapy; immunotherapy (including cancer vaccines); hormonal therapy; or radiotherapy.

Cannot have received: demethylating agent (azacitidine, decitabine)

Use of demethylating agents (azacitidine, decitabine)

Cannot have received: cytotoxic agent

Use of cytotoxic agents (including for autoimmune/inflammatory conditions)

Structured fields extracted by AI. May contain errors — verify against the official protocol.

Frequently asked questions

Is NCT07741435 currently recruiting?

Yes, this trial is currently recruiting patients.

Are there prior therapy exclusions?

Yes. Prior anticancer therapy, demethylating agent, cytotoxic agent disqualifies patients from enrollment.

Could you qualify for this trial?

Enter your biomarker results to see how this trial's eligibility criteria match your specific cancer profile.

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