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OncoMatch/Clinical Trials/NCT07359235

Safety, Tolerability, and Preliminary Efficacy of KD01 Via Intravesical Instillation in Bladder Cancer (BC)

Is NCT07359235 recruiting? Yes, currently enrolling (May 2026). This Phase 1 trial studies KD01(the recombinant oncolytic adenovirus) for bladder cancer (bc).

Phase 1RecruitingTongji HospitalNCT07359235Data as of May 2026

Treatment: KD01(the recombinant oncolytic adenovirus)Recombinant oncolytic adenovirus injection (KD01) is an oncolytic virus product. Its main component is a conditionally replicative recombinant human type 5 adenovirus, where part of the E3 region has been replaced with the gene encoding the tBid apoptotic protein.AK104 is a humanized bispecific antibody co-targeting PD-1 (Programmed Cell Death Protein 1) and CTLA-4 (Cytotoxic T-Lymphocyte-Associated Antigen 4)-two key immune checkpoint receptors. It is designed as a novel tetrameric construct that preferentially binds to tumor-infiltrating lymphocytes (TILs) co-expressing PD-1 and CTLA-4 in the tumor microenvironment (with higher avidity than in peripheral tissues).This is a Phase I clinical study designed to evaluate the safety, tolerability, and preliminary efficacy of intravesical instillation of recombinant oncolytic adenovirus injection (KD01) in patients with bladder cancer.This study consists of Phase Ia and Phase Ib, where Phase Ia is a dose-escalation stage.The Phase Ia will include histopathologically confirmed non-muscle-invasive bladder cancer (NMIBC) patients with high-risk stratification (including extremely high-risk).The Phase Ib study will include two independent cohorts: Cohort A comprises high-risk non-muscle-invasive bladder cancer (NMIBC) patients (carcinoma in situ \[CIS\], with or without Ta/T1 stage lesions) who have shown no response to BCG. Cohort B will include T2-4aN0-1M0 stage bladder cancer patients (clinically localized muscle-invasive bladder urothelial carcinoma).

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Extracted eligibility criteria

Cancer type

Urothelial Carcinoma

Disease stage

Required: Stage CIS (TNM)

Grade: G3high-grade

G3/high-grade tumors that meet any of the following criteria: carcinoma in situ (CIS); stage T1; diameter>3 cm; multiple tumors; or recurrent tumors. ... muscle-invasive bladder cancer (cT2-4aN0-1M0)

Performance status

ECOG 0–1(Restricted strenuous activity)

Prior therapy

Must have received: BCG therapy — NMIBC

BCG unresponsive NMIBC patients with carcinoma in situ [CIS], with or without Ta/T1 stage lesions

Cannot have received: pelvic external beam radiation therapy

Received pelvic external beam radiation therapy within 5 years

Cannot have received: adenovirus-based therapies (oncolytic adenovirus, CanSino's Ad5-nCoV COVID-19 vaccine)

Prior treatment with adenovirus-based therapies (e.g., oncolytic adenovirus, CanSino's Ad5-nCoV COVID-19 vaccine)

Cannot have received: anti-tumor treatments (chemotherapy, radiotherapy, biological therapy, endocrine therapy, targeted therapy, immunotherapy)

Exception: immediate postoperative instillation after TURBT allowed

patients who have received anti-tumor treatments (including chemotherapy, radiotherapy, biological therapy, endocrine therapy, targeted therapy, immunotherapy, etc.) within 4 weeks before the first study drug administration must be excluded

Cannot have received: nitrosourea drugs

patients who have used nitrosourea drugs or mitomycin C within 6 weeks prior to the initial study drug administration must be excluded

Cannot have received: alkylating agent (mitomycin C)

patients who have used nitrosourea drugs or mitomycin C within 6 weeks prior to the initial study drug administration must be excluded

Cannot have received: antimetabolite (oral fluorouracil)

patients who have used oral fluorouracil and small-molecule targeted drugs within 2 weeks prior to the first administration of the study drug or within 5 half-lives of the drugs (whichever is longer) must be excluded

Cannot have received: targeted therapy (small-molecule targeted drugs)

patients who have used oral fluorouracil and small-molecule targeted drugs within 2 weeks prior to the first administration of the study drug or within 5 half-lives of the drugs (whichever is longer) must be excluded

Cannot have received: traditional Chinese medicine with anti-tumor indications

Patients who have used TCMs with anti-tumor indications within 2 weeks prior to the first administration must be excluded

Lab requirements

Blood counts

ANC≥1.5×10^9/L; Platelets≥75×10^9/L; Hemoglobin≥90g/L

Kidney function

Creatinine (Cr)≤1.5×ULN; Creatinine clearance (Ccr)>50 mL/min if Cr>1.5×ULN

Liver function

Total bilirubin (TBIL)≤1.5× upper limit (ULN); ALT≤3×ULN; AST≤3×ULN

Cardiac function

No severe cardiac rhythm or conduction abnormalities; QTcF<460ms; No acute coronary syndrome, CHF, aortic dissection, stroke or other grade 3+ events within 6 months; NYHA class <II; LVEF≥50%; no high-risk structural heart disease; controlled hypertension

No dysfunction of major organs, including but not limited to hematopoietic function and cardiac, pulmonary, hepatic and renal function. ... Hematologic system (no history of blood transfusion or hematopoietic growth factor treatment within 14 days)... Hepatic function... Renal function... Coagulation function...

Structured fields extracted by AI. May contain errors — verify against the official protocol.

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