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OncoMatch/Clinical Trials/NCT05451784

Treatment of Advanced or Metastatic Triple-negative Breast Cancer With Adoptive Therapy of PD1+ TILS

Is NCT05451784 recruiting? Yes, currently enrolling (May 2026). This Phase 1/2 trial studies PD1+ TILs (NUMARZU-001) product infusion for metastatic triple-negative breast carcinoma.

Phase 1/2RecruitingFundacio Clinic BarcelonaNCT05451784Data as of May 2026

Treatment: PD1+ TILs (NUMARZU-001) product infusionThis is a prospective, multicenter, phase I/II, open-label, two-stage design of PD1+ TILs infusion in metastatic or advanced TNBC. TILS001 includes 3 parts. Previous to each phase inclusion, a specific ICF must be signed by the patient. Participants potentially eligible to participate in the clinical trial will be offered to sign a ICF three times prior to TILs treatment: 1) to allow for collection of archival FFPE tissue samples for determination PD1 by mRNA (Part #1), 2) prior to a fresh metastatic biopsy for selection, isolation and partial expansion of PD1+ TILs (Part #2) and 3) prior to allow for remaining study procedures and TILs therapy (Part #3, Main Consent).

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Extracted eligibility criteria

Cancer type

Triple-Negative Breast Cancer

Breast Carcinoma

Biomarker criteria

Required: PD-L1 (CD274) mRNA expression above the 20th percentile (above 20th percentile)

PD-1 mRNA expression above the 20th percentile in the FFPE tumor sample analyzed in Part 1

Required: HER2 (ERBB2) negative in situ hybridization test or IHC status of 0 or 1+ (IHC 0 or 1+ or negative ISH)

HER2-negative in situ hybridization test or an immunohistochemistry (IHC) status of 0 or 1+

Required: ESR1 expression <10% (<10%)

ER and PgR expressions <10% as determined locally by IHC assay

Required: PR (PGR) expression <10% (<10%)

ER and PgR expressions <10% as determined locally by IHC assay

Disease stage

Metastatic disease required

Histologically confirmed diagnosis of unresectable or metastatic breast cancer.

Performance status

ECOG 0–1(Restricted strenuous activity)

Prior therapy

Max 5 prior lines
Min 2 prior lines

Must have received: antibody-drug conjugate

All patients must have received two or more prior systemic therapies, including at least one of them for advanced disease and an ADC.

Cannot have received: allogeneic hematopoietic stem cell transplantation

Patients must NOT have undergone prior allogeneic hematopoietic stem cell transplantation

Lab requirements

Blood counts

Adequate organ function determined within 28 days prior to enrollment.

Kidney function

Adequate organ function determined within 28 days prior to enrollment.

Liver function

Adequate organ function determined within 28 days prior to enrollment.

Cardiac function

For patients ≥ 60 years or with cardiac history, cardiac stress test must show normal LVEF, NYHA < class 1, and any wall movement abnormalities must be reversible. LVEF ≥ 50% at baseline as determined by ECHO or MUGA.

Adequate organ function determined within 28 days prior to enrollment. For patients ≥ 60 years or patients who have a history of ischemic heart disease, chest pain, or clinically significant atrial and/or ventricular arrhythmias, a cardiac stress tests must be performed showing normal LVEF, NYHA functional classification < class 1 and if any wall movement abnormalities, they must be reversible. Left ventricular ejection fraction (LVEF) ≥ 50% at baseline as determined by either ECHO or MUGA

Structured fields extracted by AI. May contain errors — verify against the official protocol.

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