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OncoMatch/Clinical Trials/NCT03676504

Treatment of Patients With Relapsed or Refractory CD19+ Lymphoid Disease With T Cells Expressing a Third-generation CAR

Is NCT03676504 recruiting? Yes, currently enrolling (Jun 2026). This Phase 1/2 trial studies multiple treatments including CD19.CAR T Cells and Fludarabine for acute lymphoblastic leukemia, adult.

Phase 1/2RecruitingUniversity Hospital HeidelbergNCT03676504Data as of Jun 2026Location: Germany

Treatment: CD19.CAR T Cells · Fludarabine · CyclophosphamideAdult patients with r/r acute lymphoblastic leukemia (ALL) (stratum I), r/r Non-Hodgkin's lymphoma (NHL) including chronic lymphocytic leukaemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL) or mantle cell lymphoma (MCL) (stratum II) as well as paediatric patients with r/r ALL (stratum III) will be treated with autologous T-lymphocytes transduced by the third-generation RV-SFG.CD19.CD28.4-1BBzeta retroviral vector. The main purpose of this study is to evaluate safety and feasibility of escalating CD19.CAR T cell doses (0,1-20×20\^7 transduced cells/m\^2) after lymphodepletion with fludarabine and cyclophosphamide.

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Extracted eligibility criteria

Treatments studied

Chemotherapy

FludarabineCyclophosphamide

Other

CD19.CAR T Cells

Cancer type

Acute Lymphoblastic Leukemia

Chronic Lymphocytic Leukemia

Diffuse Large B-Cell Lymphoma

Non-Hodgkin Lymphoma

Biomarker criteria

Required: CD19 overexpression (confirmed CD19 expression on malignant cells in relapse)

Confirmed CD19+ ALL, CLL, DLBCL, FL or MCL by cytology and flow cytometry (FACS)

Performance status

ECOG 0–2(Ambulatory, capable of self-care)

Demographics

Ages ≥ 3

Prior therapy

Min 2 prior lines

Must have received: chemoimmunotherapy

Relapsed or refractory disease (including "molecular relapse" with minimal residual disease (MRD) levels > 10^-3 at two occasions > 2 weeks apart) with confirmed CD19 expression on malignant cells in relapse

Must have received: Bruton's tyrosine kinase inhibitor — CLL

failure or intolerance of both Bruton's tyrosine kinase Inhibitor (BTKi) and B-cell lymphoma 2 inhibitors (BCL-2i)

Must have received: B-cell lymphoma 2 inhibitor — CLL

failure or intolerance of both Bruton's tyrosine kinase Inhibitor (BTKi) and B-cell lymphoma 2 inhibitors (BCL-2i)

Must have received: autologous stem cell transplantation — DLBCL, FL, MCL

Relapse after autologous stem cell transplantation (autoSCT)

Must have received: allogeneic stem cell transplantation — ALL, CLL, DLBCL, FL, MCL

Any relapse after allogeneic stem cell transplantation (alloSCT) (≥ 6 months from alloSCT at time of CAR T cell infusion)

Must have received: idelalisib — FL

ineligibility for or failure of idelalisib

Lab requirements

Blood counts

ANC ≥ 500/mm3; ALC ≥ 100/mm3

Kidney function

serum creatinine of ≤ 2 x ULN or eGFR ≥ 30 mL/min/1.73 m^2 (adults); serum creatinine-clearance ≥ 30 mL/min/1.73 m^2 (children)

Liver function

ALT ≤ 5 times the ULN for the respective age; Bilirubin ≤ 2.0 mg/dl (exceptions for Gilbert-Meulengracht syndrome or extrahepatic disease)

Cardiac function

Hemodynamic stability and LVEF ≥ 40% (adults); LVEF ≥ 40% or shortening fraction > 29% (children)

Adequate organ function: Renal function defined as: serum creatinine of ≤ 2 x ULN or estimated glomerular filtration rate (eGFR) ≥ 30 mL/min/1.73 m^2; Liver function defined as: ALT ≤ 5 times the ULN for the respective age; Bilirubin ≤ 2.0 mg/dl with the exception of patients with hyperbilirubinemia explained by Gilbert-Meulengracht syndrome (may be included if total bilirubin is ≤ 3.0 x ULN and direct bilirubin ≤ 1.5 x ULN) or extrahepatic disease (e.g. chronic hemolytic anemia); minimum level of pulmonary reserve defined as ≤ grade 1 dyspnea and pulse oxygenation > 90% on room air; Hemodynamic stability and left ventricular ejection fraction (LVEF) ≥ 40% as confirmed by echocardiogram; Absolute neutrophil count (ANC) ≥ 500/mm3; Absolute lymphocyte count (ALC) ≥ 100/mm3

Structured fields extracted by AI. May contain errors — verify against the official protocol.

Frequently asked questions

Is NCT03676504 currently recruiting?

Yes, this trial is currently recruiting patients.

Is prior treatment required for enrollment?

Yes. Patients must have previously received chemoimmunotherapy and Bruton's tyrosine kinase inhibitor.

Does this trial require CD19?

Yes, CD19 overexpression is a required biomarker for enrollment.

Could you qualify for this trial?

Enter your biomarker results to see how this trial's eligibility criteria match your specific cancer profile.

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