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OncoMatch/Clinical Trials/NCT02422641

Prospective Evaluation Of High-Dose Systemic Methotrexate In Patients With Breast Cancer And Leptomeningeal Metastasis

Is NCT02422641 recruiting? Yes, currently enrolling (Jun 2026). This Phase 2 trial studies High-dose Methotrexate (8 gm/m2; HD-MTX) for metastatic breast cancer.

Phase 2RecruitingWake Forest University Health SciencesNCT02422641Data as of Jun 2026

Treatment: High-dose Methotrexate (8 gm/m2; HD-MTX)This study is a prospective evaluation of systemic, intravenous high-dose methotrexate (HD-MTX, 8 g/m2) in patients with triple negative, HER2-positive, and hormone refractory breast cancer with leptomeningeal metastasis (LMD) with or without brain parenchymal involvement.

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Extracted eligibility criteria

Treatments studied

Other

High-dose Methotrexate (8 gm/m2; HD-MTX)

Cancer type

Breast Carcinoma

Biomarker criteria

Required: ESR1 negative (ER-negative)

TRIPLE NEGATIVE (ER-negative, PR-negative, and HER2-negative disease)

Required: PR (PGR) negative (PR-negative)

TRIPLE NEGATIVE (ER-negative, PR-negative, and HER2-negative disease)

Required: HER2 (ERBB2) negative (HER2-negative)

TRIPLE NEGATIVE (ER-negative, PR-negative, and HER2-negative disease)

Required: HER2 (ERBB2) overexpression (HER2-positive)

HER2-POSITIVE: HER2-positive patients will be defined per ASCO-CAP Guidelines.

Allowed: ESR1 positive

HORMONE REFRACTORY: Patients with ER/PR-positive disease... may be considered if they have disease progression after two lines of hormonal therapy... or are deemed clinically hormone-resistant...

Allowed: PR (PGR) positive

HORMONE REFRACTORY: Patients with ER/PR-positive disease... may be considered if they have disease progression after two lines of hormonal therapy... or are deemed clinically hormone-resistant...

Disease stage

Metastatic disease required

Performance status

ECOG 0–1(Restricted strenuous activity)

Prior therapy

Must have received: hormonal therapy — adjuvant or metastatic

disease progression after two lines of hormonal therapy (administered in the adjuvant or metastatic setting), or are deemed clinically hormone-resistant

Cannot have received: methotrexate containing systemic regimen (methotrexate)

Exception: excluding intrathecal methotrexate

Prior treatment with any methotrexate containing systemic regimen within 1 year (excluding intrathecal methotrexate)

Cannot have received: chemotherapy

Chemotherapy... within the last 2 weeks

Cannot have received: stereotactic radiotherapy

stereotactic radiotherapy within the last 2 weeks

Cannot have received: whole brain radiotherapy

Whole brain radiotherapy within the last 6 months

Cannot have received: partial brain radiotherapy

Exception: partial brain radiotherapy <40% of total brain volume within the last 2 weeks; partial brain radiotherapy >40% of total brain volume within the last 6 months

Partial brain radiotherapy (i.e. <40% of total brain volume) within the last 2 weeks; partial brain radiotherapy exceeding >40% of total brain volume within the last 6 months

Lab requirements

Blood counts

White blood cell counts >3000 cells/mcL; Absolute neutrophil count >1500 cells/mcL; Platelet count >100,000 cells/mcL; Hematocrit >30%

Kidney function

Estimated creatinine clearance >70 cc/min (Cockcroft-Gault formula)

Liver function

Serum bilirubin <1.5 x the ULN or <5x the ULN if secondary to liver metastasis; ALT or AST <2.5x the ULN or <5x the ULN if secondary to liver metastasis; Alkaline phosphatase <2.5x the ULN or <5x the ULN if secondary to liver metastasis

Adequate organ function as follows: Estimated creatinine clearance >70 cc/min (calculated by Cockcroft-Gault formula) White blood cell counts >3000 cells/mcL Absolute neutrophil count >1500 cells/mcL Platelet count >100,000 cells/mcL Hematocrit >30% Serum bilirubin <1.5 x the ULN or <5x the ULN if secondary to liver metastasis Alanine aminotransferase or aspartate aminotransferase <2.5x the ULN or <5x the ULN if secondary to liver metastasis Alkaline phosphatase <2.5x the ULN or <5x the ULN if secondary to liver metastasis

Structured fields extracted by AI. May contain errors — verify against the official protocol.

US trial sites

  • Sidney Kimmel Comprehensive Cancer Center · Baltimore, Maryland
  • Siteman Cancer Center- Washington University School of Medicine in St. Louis · St Louis, Missouri
  • Comprehensive Cancer Center at Wake Forest University (CCCWFU) · Winston-Salem, North Carolina

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See all sites on ClinicalTrials.gov →

Frequently asked questions

Is NCT02422641 currently recruiting?

Yes, this trial is currently recruiting patients.

Are there prior therapy exclusions?

Yes. Prior methotrexate containing systemic regimen, chemotherapy, stereotactic radiotherapy disqualifies patients from enrollment.

Does this trial require ESR1?

Yes, ESR1 negative is a required biomarker for enrollment.

Does this trial require PGR?

Yes, PGR negative is a required biomarker for enrollment.

Does this trial require ERBB2?

Yes, ERBB2 negative is a required biomarker for enrollment.

Could you qualify for this trial?

Enter your biomarker results to see how this trial's eligibility criteria match your specific cancer profile.

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